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Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
Human VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit
The Human (VEGF-A) Vascular Endothelial Cell Growth Factor A ELISA Kit measures Vascular Endothelial Cell Growth Factor A in samples. The plate has been pre-coated with Human VEGFA antibody. VEGFA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VEGFA Antibody is added and binds to VEGFA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VEGFA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VEGFA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0050Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
1.52 ng/L
Detection Range
3-900 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cancer, Cardiovascular
Background
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Vascular Endothelial Cell Growth Factor A
Regulatory Status
For Research Use Only
