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Human VIM (Vimentin) ELISA Kit

Human VIM (Vimentin) ELISA Kit

The Human (VIM) Vimentin ELISA Kit measures Vimentin in samples. The plate has been pre-coated with Human VIM antibody. VIM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human VIM Antibody is added and binds to VIM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VIM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human VIM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1673Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.07 ng/mL
Detection Range 0.2-60 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cancer
Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Vimentin
Regulatory Status For Research Use Only
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