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Human WNK2 (Serine Threonine-protein Kinase Wnk2) ELISA Kit
Human WNK2 (Serine Threonine-protein Kinase Wnk2) ELISA Kit
The Human (WNK2) Serine Threonine-protein Kinase Wnk2 ELISA Kit measures Serine/threonine-protein kinase WNK2 in samples. The plate has been pre-coated with Human WNK2 antibody. WNK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNK2 Antibody is added and binds to WNK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human WNK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6149Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
7.55 ng/L
Detection Range
15-3000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Enzyme & Kinase
Background
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SLC12A2, SCNN1A, SCNN1B, SCNN1D and SGK1 and inhibits SLC12A5. Negatively regulates the EGF-induced activation of the ERK/MAPK-pathway and the downstream cell cycle progression. Affects MAPK3/MAPK1 activity by modulating the activity of MAP2K1 and this modulation depends on phosphorylation of MAP2K1 by PAK1. WNK2 acts by interfering with the activity of PAK1 by controlling the balance of the activity of upstream regulators of PAK1 activity, RHOA and RAC1, which display reciprocal activity. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Serine/threonine-protein kinase WNK2
Regulatory Status
For Research Use Only
