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Human WNT3A (Protein Wnt-3A) ELISA Kit

Human WNT3A (Protein Wnt-3A) ELISA Kit

The Human (WNT3A) Protein Wnt-3A ELISA Kit measures Protein Wnt-3A in samples. The plate has been pre-coated with Human WNT3A antibody. WNT3A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT3A Antibody is added and binds to WNT3A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WNT3A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human WNT3A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2349Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.02 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Stem Cells
Background Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed: 20093360, PubMed: 21244856, PubMed: 24841207, PubMed: 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed: 26902720). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Protein Wnt-3A
Regulatory Status For Research Use Only
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