The Human (WWTR1) Ww Domain-containing Transcription Regulator Protein 1 ELISA Kit measures Ww Domain-Containing Transcription Regulator Protein 1 in samples. The plate has been pre-coated with Human WWTR1 antibody. WWTR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WWTR1 Antibody is added and binds to WWTR1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated WWTR1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human WWTR1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundTranscriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed: 11118213, PubMed: 18227151). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed: 18227151). WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation (PubMed: 19010321). In conjunction with YAP1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (PubMed: 18568018). Plays a key role in coupling SMADs to the transcriptional machinery such as the mediator complex (PubMed: 18568018). Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition (PubMed: 18227151, PubMed: 18568018). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteWw Domain-Containing Transcription Regulator Protein 1
