Human XCL1/Lptn/LTN (Lymphotactin) ELISA Kit
Human XCL1/Lptn/LTN (Lymphotactin) ELISA Kit
The Human XCL1/Lptn/LTN (Lymphotactin) ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Lptn/LTN/XCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Lptn/LTN/XCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Lptn/LTN/XCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Lptn/LTN/XCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
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