• KL-6 ELISA
1 1

KL-6 ELISA

KL-6 ELISA

The KL-6 ELISA is an enzyme immunoassay for the quantitative determination of KL-6 in human serum, plasma and bronchoalveolar lavage fluid (BALF).

The microtiter plate is coated with the antibody specifically binding the KL-6. The human serum, plasma or BALF is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of KL-6 in the specimen. The concentration of KL-6 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1017
$560.00 USD
$560.00 USD $560.00 USD
Sale Sold out
Packaging
2 weeks

Product Details

Species Reactivity Human
Sensitivity 7.8 U/mL
Detection Range 0.156 - 10 U/mL
Sample Type Serum, Plasma
Sample Size 5 uL
Incubation(s) 2.5 hour(s)
Research Areas Pulmonary
Background Interstitial lung diseases (ILD) are a group of lung disorders characterized by interstitial lung thickening. Krebs von den Lungen-6 (KL-6) is a 200 kDa glycoprotein that is predominantly expressed by damaged alveolar type II cells, and it has been proposed as a potential biomarker of different ILD. KL-6 has profibrotic and anti-apoptotic effects on lung fibroblasts and reflects the degree and severity of alveolar epithelial injury. KL-6 is predictive biomarker useful in the management of ILD, particularly with severe fibrotic lung disorders. Serum KL-6 levels were also found elevated in Rheumatoid Arthritis – Associated IDL, and the levels positively correlated with HRCT fibrosis score.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte KL-6
Regulatory Status For Research Use Only
View full details

Inquire about this product