LTF (Lactoferrin) ELISA Kit
LTF (Lactoferrin) ELISA Kit
This LTF (Lactoferrin) ELISA Kit uses the sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human LTF are added to the micro ELISA plate wells. Human LTF in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human LTF.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human LTF. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
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