Monkey Anti-FLAG-tag Antibody ELISA Kit
Monkey Anti-FLAG-tag Antibody ELISA Kit
The Monkey Anti-FLAG-tag Antibody ELISA Kit measures anti-FLAG-tag Ab in Monkey Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antigen, then it binds with anti-FLAG-tag Ab bound to precoated antigen. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-FLAG-tag Ab in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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