The monomeric CRP (mCRP) ELISA is an enzyme immunoassay for the quantitative determination of monomeric CRP (mCRP) in human serum and plasma.
Human Monomeric ELISA specifically determines monomeric CRP (mCRP) without any reactivity with pentameric CRP (pCRP).
The microtiter plate is coated with the antibody specifically binding the mCRP. The human serum and plasma are incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of mCRP in the specimen. The concentration of mCRP in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
Catalog No:BA1026
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Product Details
Species ReactivityHuman
Sensitivity0.63 ng/mL
Detection Range12.5 - 800 ng/mL
Sample TypeSerum, Plasma
Sample Size80 uL
Incubation(s)ON + 2.5 hour(s)
Research AreasCardiovascular
BackgroundC-reactive protein (CRP) is a multipotent protein that undergoes conformational changes between circulating native pentameric CRP (pCRP), pentameric symmetrical forms (pCRP*) and monomeric CRP (mCRP) forms. mCRP exhibits strong pro-inflammatory activity and activates platelets, leukocytes, and endothelial cells. Abundant deposition of mCRP in inflamed tissues plays a role in several disease conditions, such as ischemia/reperfusion injury, Alzheimer’s disease, and cardiovascular disease.
Conversion of pCRP to mCRP induces inflammatory signalling, monoacyl phosphatidylcholine generated by PLA2, or by oxidation lipid acyl chains, promotes binding and dissociation of pCRP to mCRP. mCRP gains functionally active neoepitopes that carry out highly pro-inflammatory and pro-thrombotic features, thus mCRP can bind cholesterol and enter plasmatic membrane and activate pro-inflammatory responses.
Deposition of mCRP, which has a much lower aqueous solubility than pCRP, has been shown in the brain in infarcted areas of Alzheimer diseases patients and in regions with amyloid burden, in atherosclerotic plaques in vascular disease and in other foci of inflammatory tissue injuries.
