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Mouse 5-NT (5-nucleotidase) ELISA Kit

Mouse 5-NT (5-nucleotidase) ELISA Kit

The Mouse (5-NT) 5-nucleotidase ELISA Kit measures 5-Nucleotidase in Mouse samples. The plate has been pre-coated with Mouse 5-NT antibody. 5-NT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse 5-NT Antibody is added and binds to 5-NT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated 5-NT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse 5-NT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0083Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.033 mIU/mL
Detection Range 0.06-18 mIU/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cardiovascular
Background Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte 5-Nucleotidase
Regulatory Status For Research Use Only
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