• Mouse a1AT (Alpha-1-Antitrypsin) ELISA Immunoassay Kit
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Mouse a1AT (Alpha-1-Antitrypsin) ELISA Kit (Small Sample)

Mouse a1AT (Alpha-1-Antitrypsin) ELISA Kit (Small Sample)

The Mouse a1AT (Alpha-1-Antitrypsin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse a1AT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a1AT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse a1AT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a1AT in the samples is then determined by comparing the OD of the samples to the standard curve.

Catalog No: ELK11110MS
$595.40 USD
$458.00 USD $595.40 USD
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Product Details

Species Reactivity Mouse
Sensitivity 5 µg/mL
Detection Range 15.63-1000 µg/mL
Sample Type Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Incubation(s) 3.5 hour(s)
Research Areas Cell Biology, Enzyme & Kinase, Gastroenterology, Signal Transduction
Shipping Condition Shipped on cold gel packs.
Analyte SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1
Regulatory Status For Research Use Only
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