The Mouse (ABCD1) Atp Binging Cassette Sub Family D Member 1 ELISA Kit measures Atp Binging Cassette Sub Family D Member 1 in Mouse samples. The plate has been pre-coated with Mouse ABCD1 antibody. ABCD1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ABCD1 Antibody is added and binds to ABCD1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCD1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ABCD1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPlays a role in the transport of free very-long-chain fatty acids (VLCFAs) as well as their CoA-esters across the peroxisomal membrane by acting as an ATP-specific binding subunit releasing ADP after ATP hydrolysis (By similarity). Thus, plays a role in regulation of VLCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation, mitochondrial function and microsomal fatty acid elongation (PubMed: 25255441, PubMed: 9418970, PubMed: 9126326, PubMed: 9256488, PubMed: 18854420, PubMed: 23123468, PubMed: 26108493, PubMed: 23604518, PubMed: 25583114). Involved in several processes; namely, controls the active myelination phase by negatively regulating the microsomal fatty acid elongation activity and may also play a role in axon and myelin maintenance (PubMed: 11875044, PubMed: 26108493, PubMed: 15489218). Controls also the cellular response to oxidative stress by regulating mitochondrial function like, mitochondrial oxidative phosphorylation and depolarization (PubMed: 25583114, PubMed: 23604518, PubMed: 18344354, PubMed: 22521832). And finally controls the inflammatory response by positively regulating peroxisomal beta-oxidation of VLCFAs (PubMed: 18723473). Source: UniProt Consortium (2025)
