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Mouse ACACA (Acetyl-CoA Carboxylase 1) ELISA Kit
Mouse ACACA (Acetyl-CoA Carboxylase 1) ELISA Kit
The Mouse (ACACA) Acetyl-CoA Carboxylase 1 ELISA Kit measures Acetyl-Coa Carboxylase 1 in Mouse samples. The plate has been pre-coated with Mouse ACACA antibody. ACACA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ACACA Antibody is added and binds to ACACA in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ACACA antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ACACA. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1228Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
2.69 ng/L
Detection Range
5-1000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed: 20952656). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed: 20952656). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Acetyl-Coa Carboxylase 1
Regulatory Status
For Research Use Only
