Mouse ACTH (Corticotropin) ELISA Kit
Mouse ACTH (Corticotropin) ELISA Kit
This ACTH ELISA kit is based on Competitive ELISA detection method and takes approximately 2 hours. The microplate provided in this kit has been precoated with ACTH. ACTH in the sample or standard competes with a fixed amount of biotin-labeled ACTH for sites on a pre-coated antibody specific to ACTH. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of ACTH in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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