The Mouse (ADAM15) Disintegrin and Metalloproteinase Domain-containing Protein 15 ELISA Kit measures Disintegrin and metalloproteinase domain-containing protein 15 in Mouse samples. The plate has been pre-coated with Mouse ADAM15 antibody. ADAM15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ADAM15 Antibody is added and binds to ADAM15 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAM15 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ADAM15. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundActive metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration (By similarity).
Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. Interactions with egg membrane could be mediated via binding between the disintegrin-like domain to one or more integrin receptors on the egg. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteDisintegrin and metalloproteinase domain-containing protein 15
