The Mouse (ADAM17) Disintegrin and Metalloproteinase Domain-containing Protein 17 ELISA Kit measures Disintegrin and metalloproteinase domain-containing protein 17 in Mouse samples. The plate has been pre-coated with Mouse ADAM17 antibody. ADAM17 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ADAM17 Antibody is added and binds to ADAM17 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ADAM17 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ADAM17. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Plays a role in the proteolytic processing of ACE2 (By similarity).
Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:10799547, PubMed:11108241).
Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called notch extracellular truncation (NEXT) (PubMed:10882063).
Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain (PubMed:12907434).
Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3 (PubMed:17245433).
Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R (By similarity). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteDisintegrin and metalloproteinase domain-containing protein 17
