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Mouse ALDH4A1 (Delta-1-pyrroline-5-carboxylate Dehydrogenase) ELISA Kit

Mouse ALDH4A1 (Delta-1-pyrroline-5-carboxylate Dehydrogenase) ELISA Kit

The Mouse (ALDH4A1) Delta-1-pyrroline-5-carboxylate Dehydrogenase ELISA Kit measures Delta-1-pyrroline-5-carboxylate dehydrogenase in Mouse samples. The plate has been pre-coated with Mouse ALDH4A1 antibody. ALDH4A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ALDH4A1 Antibody is added and binds to ALDH4A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ALDH4A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ALDH4A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1585Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 5.18 ng/L
Detection Range 10-3000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Tags and Cell Markers
Background Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Delta-1-pyrroline-5-carboxylate dehydrogenase
Regulatory Status For Research Use Only
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