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Mouse ALDOB (Fructose-bisphosphate Aldolase B) ELISA Kit
Mouse ALDOB (Fructose-bisphosphate Aldolase B) ELISA Kit
The Mouse (ALDOB) Fructose-bisphosphate Aldolase B ELISA Kit measures Fructose-bisphosphate aldolase B in Mouse samples. The plate has been pre-coated with Mouse ALDOB antibody. ALDOB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ALDOB Antibody is added and binds to ALDOB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ALDOB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ALDOB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2080Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.155 ng/mL
Detection Range
0.3-90 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Aldolase B also known as fructose-bisphosphate aldolase B or liver-type aldolase is one of three isoenzymes (A, B, and C) of the class I fructose 1,6-bisphosphate aldolase enzyme (EC 4.1.2.13), and plays a key role in both glycolysis and gluconeogenesis. The generic fructose 1,6-bisphosphate aldolase enzyme catalyzes the reversible cleavage of fructose 1,6-bisphosphate (FBP) into glyceraldehyde 3-phosphate and dihydroxyacetone phosphate (DHAP) as well as the reversible cleavage of fructose 1-phosphate (F1P) into glyceraldehyde and dihydroxyacetone phosphate. In mammals, aldolase B is preferentially expressed in the liver, while aldolase A is expressed in muscle and erythrocytes and aldolase C is expressed in the brain. Slight differences in isozyme structure result in different activities for the two substrate molecules: FBP and fructose 1-phosphate. Aldolase B exhibits no preference and thus catalyzes both reactions, while aldolases A and C prefer FBP. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Fructose-bisphosphate aldolase B
Regulatory Status
For Research Use Only
