Mouse Anti-TNF-Alpha (Tumor Necrosis Factor Alpha) Antibody ELISA Kit
Mouse Anti-TNF-Alpha (Tumor Necrosis Factor Alpha) Antibody ELISA Kit
The Mouse Anti-TNF-Alpha (Tumor Necrosis Factor Alpha) Antibody ELISA Kit measures Anti-TNF-Alpha in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with Anti-TNF-α bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Anti-TNF-α in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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