The Mouse (ARNT) Aryl Hydrocarbon Receptor Nuclear Translocator ELISA Kit measures Aryl Hydrocarbon Receptor Nuclear Translocator in Mouse samples. The plate has been pre-coated with Mouse ARNT antibody. ARNT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ARNT Antibody is added and binds to ARNT in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ARNT antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ARNT. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundRequired for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity).
The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878).
The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820). Source: UniProt Consortium (2025)
