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Mouse ATG4B (Autophagy Related Protein 4B) ELISA Kit
Mouse ATG4B (Autophagy Related Protein 4B) ELISA Kit
The Mouse (ATG4B) Autophagy Related Protein 4B ELISA Kit measures Autophagy Related Protein 4B in Mouse samples. The plate has been pre-coated with Mouse ATG4B antibody. ATG4B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ATG4B Antibody is added and binds to ATG4B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATG4B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ATG4B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2636Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.024 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cancer, Cell Biology
Background
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Autophagy Related Protein 4B
Regulatory Status
For Research Use Only
