Mouse BSA-IgE (Bovine serum albumin IgE) ELISA kit
Mouse BSA-IgE (Bovine serum albumin IgE) ELISA kit
The Mouse BSA-IgE (Bovine serum albumin IgE) ELISA kit measures BSA-IgE in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Biotinylated antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with BSA-IgE bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of BSA-IgE in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
Couldn't load pickup availability
Product Details
