The Mouse (BUB1B) Mitotic Checkpoint Serine Threonine-protein Kinase Bub1 Beta ELISA Kit measures Mitotic checkpoint serine/threonine-protein kinase BUB1 beta in Mouse samples. The plate has been pre-coated with Mouse BUB1B antibody. BUB1B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse BUB1B Antibody is added and binds to BUB1B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BUB1B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse BUB1B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundEssential component of the mitotic checkpoint. Required for normal mitosis progression and tumor suppression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. Essential for tumor suppression. May play a role in regulating aging and fertility (By similarity). Source: UniProt Consortium (2025)
