This ELISA kit is designed to detect Mouse Carbonic Anhydrase-Related Protein (Mouse CA8). The assay plate has been pre-coated with mouse anti-Mouse Carbonic Anhydrase 8 monoclonal antibody. When the sample containing Carbonic Anhydrase 8 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse Carbonic Anhydrase 8 Antibody is added to the wells and binds to Carbonic Anhydrase 8 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse Carbonic Anhydrase 8 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
BackgroundThe carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
