The Mouse (CD247) T-cell Surface Glycoprotein CD3 Zeta Chain ELISA Kit measures T-Cell Surface Glycoprotein Cd3 Zeta Chain in Mouse samples. The plate has been pre-coated with Mouse CD247 antibody. CD247 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CD247 Antibody is added and binds to CD247 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD247 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CD247. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPart of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN). Source: UniProt Consortium (2025)
