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Mouse CFH (Complement Factor H) ELISA Kit
Mouse CFH (Complement Factor H) ELISA Kit
The Mouse (CFH) Complement Factor H ELISA Kit measures Complement Factor H in Mouse samples. The plate has been pre-coated with Mouse CFH antibody. CFH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CFH Antibody is added and binds to CFH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CFH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CFH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0592Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
9.76 ng/mL
Detection Range
20-4500 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology
Background
Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Complement Factor H
Regulatory Status
For Research Use Only
