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Mouse CHRM2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit

Mouse CHRM2 (Muscarinic Acetylcholine Receptor M2) ELISA Kit

The Mouse (CHRM2) Muscarinic Acetylcholine Receptor M2 ELISA Kit measures Muscarinic acetylcholine receptor M2 in Mouse samples. The plate has been pre-coated with Mouse CHRM2 antibody. CHRM2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse CHRM2 Antibody is added and binds to CHRM2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CHRM2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse CHRM2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2273Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.059 ng/mL
Detection Range 0.1-40 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Neuroscience
Background The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Muscarinic acetylcholine receptor M2
Regulatory Status For Research Use Only
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