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Mouse CPLA2 (Cytosolic Phospholipase A2) ELISA Kit
Mouse CPLA2 (Cytosolic Phospholipase A2) ELISA Kit
The Mouse (CPLA2) Cytosolic Phospholipase A2 ELISA Kit measures Cytosolic Phospholipase A2 in Mouse samples. The plate has been pre-coated with Mouse PLA2G4A antibody. PLA2G4A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse PLA2G4A Antibody is added and binds to PLA2G4A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G4A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse PLA2G4A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0362Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
14.28 ng/L
Detection Range
30-9000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Has primarily calcium-dependent phospholipase and lysophospholipase activities, with a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response (PubMed: 1904318, PubMed: 9403692, PubMed: 9403693). Plays an important role in embryo implantation and parturition through its ability to trigger prostanoid production (PubMed: 9403692, PubMed: 9403693). Preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Selectively hydrolyzes sn-2 arachidonoyl group from membrane phospholipids, providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. In an alternative pathway of eicosanoid biosynthesis, hydrolyzes sn-2 fatty acyl chain of eicosanoid lysophopholipids to release free bioactive eicosanoids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 position of phospholipids (phospholipase A1 activity) only if an ether linkage rather than an ester linkage is present at the sn-2 position. This hydrolysis is not stereospecific. Has calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. Has O-acyltransferase activity. Catalyzes the transfer of fatty acyl chains from phospholipids to a primary hydroxyl group of glycerol (sn-1 or sn-3), potentially contributing to monoacylglycerol synthesis (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Cytosolic Phospholipase A2
Regulatory Status
For Research Use Only
