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Mouse EBI3 (Interleukin-27 Subunit Beta) ELISA Kit

Mouse EBI3 (Interleukin-27 Subunit Beta) ELISA Kit

The Mouse (EBI3) Interleukin-27 Subunit Beta ELISA Kit measures Interleukin-27 Subunit Beta in Mouse samples. The plate has been pre-coated with Mouse EBI3 antibody. EBI3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse EBI3 Antibody is added and binds to EBI3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated EBI3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse EBI3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2189Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.26 ng/L
Detection Range 0.5-200 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cytokines, Inflammation, Immunology
Background Associates with IL27 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interleukin-27 Subunit Beta
Regulatory Status For Research Use Only
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