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Mouse Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit

Mouse Epithelial Cell Adhesion Molecule (EPCAM) ELISA Kit

This ELISA kit is designed to detect Mouse Epithelial Cell Adhesion Molecule (Mouse EPCAM). The assay plate has been pre-coated with mouse anti-Mouse EpCAM monoclonal antibody. When the sample containing EpCAM is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Mouse EpCAM Antibody is added to the wells and binds to EpCAM in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Mouse EpCAM present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE327
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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2 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 15.63 pg/mL
Detection Range 78.13-5000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Tags and Cell Markers
Background Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â…transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Epithelial cell adhesion molecule
Regulatory Status For Research Use Only
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