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Mouse FBXO9 (F-Box Only Protein 9) ELISA Kit
Mouse FBXO9 (F-Box Only Protein 9) ELISA Kit
The Mouse (FBXO9) F-Box Only Protein 9 ELISA Kit measures F-Box Only Protein 9 in Mouse samples. The plate has been pre-coated with Mouse FBXO9 antibody. FBXO9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FBXO9 Antibody is added and binds to FBXO9 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FBXO9 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FBXO9. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1713Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
14.75 ng/L
Detection Range
30-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1 (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
F-Box Only Protein 9
Regulatory Status
For Research Use Only
