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Mouse FN3K (Fructosamine-3-kinase) ELISA Kit
Mouse FN3K (Fructosamine-3-kinase) ELISA Kit
The Mouse (FN3K) Fructosamine-3-kinase ELISA Kit measures Fructosamine-3-Kinase in Mouse samples. The plate has been pre-coated with Mouse FN3K antibody. FN3K present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse FN3K Antibody is added and binds to FN3K in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated FN3K antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse FN3K. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1354Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
14.36 ng/L
Detection Range
30-9000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Enzyme & Kinase
Background
Fructosamine-3-kinase involved in protein deglycation by mediating phosphorylation of fructoselysine residues on glycated proteins, to generate fructoselysine-3 phosphate (PubMed: 11016445, PubMed: 16819943). Fructoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (By similarity). Involved in intracellular deglycation in erythrocytes and pancreatic islets (PubMed: 16819943, PubMed: 20009024). Involved in the response to oxidative stress by mediating deglycation of NFE2L2/NRF2, glycation impairing NFE2L2/NRF2 function (PubMed: 31398338). Also able to phosphorylate psicosamines and ribulosamines (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Fructosamine-3-Kinase
Regulatory Status
For Research Use Only
