1
/
of
1
Mouse GZMS-B (Granzymes B) ELISA Kit
Mouse GZMS-B (Granzymes B) ELISA Kit
The Mouse (GZMS-B) Granzymes B ELISA Kit measures Granzymes B in Mouse samples. The plate has been pre-coated with Mouse GZMB antibody. GZMB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GZMB Antibody is added and binds to GZMB in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GZMB antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse GZMB. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0437Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Mouse
Sensitivity
3.26 ng/L
Detection Range
7-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology
Background
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Granzymes B
Regulatory Status
For Research Use Only
