1
/
of
1
Mouse HIF-1A (Hypoxia-inducible Factor 1Α) ELISA Kit
Mouse HIF-1A (Hypoxia-inducible Factor 1Α) ELISA Kit
The Mouse (HIF-1A) Hypoxia-inducible Factor 1Α ELISA Kit measures Hypoxia-Inducible Factor 1 alpha in Mouse samples. The plate has been pre-coated with Mouse HIF1A antibody. HIF1A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse HIF1A Antibody is added and binds to HIF1A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF1A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse HIF1A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0294Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Mouse
Sensitivity
0.153 ng/L
Detection Range
0.3-90 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed: 26245371). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Hypoxia-Inducible Factor 1 alpha
Regulatory Status
For Research Use Only
