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Mouse IL-17F (Interleukin 17F) ELISA Kit
Mouse IL-17F (Interleukin 17F) ELISA Kit
The Mouse (IL-17F) Interleukin 17F ELISA Kit measures Interleukin 17F in Mouse samples. The plate has been pre-coated with Mouse IL17F antibody. IL17F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL17F Antibody is added and binds to IL17F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL17F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IL17F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1183Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.53 ng/L
Detection Range
1-400 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, Cytokines, Inflammation
Background
Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed: 23255360, PubMed: 18025225, PubMed: 19144317). IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial Peptides and Matrix Metalloproteinases, with potential strong immune inflammation (PubMed: 17911633, PubMed: 15477493, PubMed: 18025225). IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation (PubMed: 18025225, PubMed: 23255360). As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (PubMed: 18025225). Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (PubMed: 19144317). IL17F homodimer can signal via IL17RC homodimeric receptor complex, triggering downstream activation of TRAF6 and NF-kappa-B signaling pathway (PubMed: 28813677). Via IL17RC induces transcriptional activation of IL33, a potent cytokine that stimulates group 2 innate lymphoid cells and adaptive T-helper 2 cells involved in pulmonary allergic response to fungi (PubMed: 28813677). Likely via IL17RC, promotes sympathetic innervation of peripheral organs by coordinating the communication between gamma-delta T cells and parenchymal cells. Stimulates sympathetic innervation of thermogenic adipose tissue by driving TGFB1 expression (PubMed: 32076265). Regulates the composition of intestinal microbiota and immune tolerance by inducing antimicrobial proteins that specifically control the growth of commensal Firmicutes and Bacteroidetes (PubMed: 29915298). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Interleukin 17F
Regulatory Status
For Research Use Only
