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Mouse IL-18 (Interleukin 18) ELISA Kit

Mouse IL-18 (Interleukin 18) ELISA Kit

The Mouse (IL-18) Interleukin 18 ELISA Kit measures Interleukin 18 in Mouse samples. The plate has been pre-coated with Mouse IL18 antibody. IL18 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IL18 Antibody is added and binds to IL18 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL18 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IL18. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0044Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.66 ng/L
Detection Range 10-600 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology, Cytokines, Inflammation
Background A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interleukin 18
Regulatory Status For Research Use Only
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