• Mouse Insulin ELISA, Time 3.5 hours Sensitivity 0.057 ng/mL
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Mouse INS (Insulin) ELISA Kit

Mouse INS (Insulin) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse INS in the samples is then determined by comparing the OD of the samples to the standard curve.

Catalog No: ELK10344
$595.00 USD
$458.00 USD $595.00 USD
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Product Details

Species Reactivity Mouse
Sensitivity 0.057 ng/mL
Detection Range 0.16-10 ng/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Incubation(s) 3.5h
Research Areas Metabolic pathway, Endocrinology, Hormone metabolism
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life If the kit is not opened, store the whole kit: 4C(short time storage, valid for 6 months); -20C(long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles If the kit is opened, Store the whole kit at 4C. If the kit is not used up in 1 week. Store the Pre-Coated Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20C, the rest reagents at 4C, please used up within 6 months.
Analyte Insulin Kits
Regulatory Status For Research Use Only
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