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Mouse IRS-1 (Insulin Receptor Substrate 1) ELISA Kit

Mouse IRS-1 (Insulin Receptor Substrate 1) ELISA Kit

The Mouse (IRS-1) Insulin Receptor Substrate 1 ELISA Kit measures Insulin Receptor Substrate 1 in Mouse samples. The plate has been pre-coated with Mouse IRS1 antibody. IRS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse IRS1 Antibody is added and binds to IRS1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IRS1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse IRS1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1163Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.031 ng/mL
Detection Range 0.05-30 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Diabetes Research, Endocrinology, Metabolism
Background May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Insulin Receptor Substrate 1
Regulatory Status For Research Use Only
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