The Mouse (LAMP2) Lysosome-associated Membrane Glycoprotein 2 ELISA Kit measures Lysosome-Associated Membrane Glycoprotein 2 in Mouse samples. The plate has been pre-coated with Mouse LAMP2 antibody. LAMP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LAMP2 Antibody is added and binds to LAMP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LAMP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse LAMP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPlays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed: 10972293). Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed: 27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed: 27628032). Required for normal degradation of the contents of autophagosomes (PubMed: 10972293, PubMed: 12221139). Plays a role in lysosomal protein degradation in response to starvation (PubMed: 27628032). Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic Peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits. Is not required for efficient MHCII-mediated presentation of endogenous antigens (By similarity). Source: UniProt Consortium (2025)
