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Mouse LEF1 (Lymphoid Enhancer-binding Factor 1) ELISA Kit
Mouse LEF1 (Lymphoid Enhancer-binding Factor 1) ELISA Kit
The Mouse (LEF1) Lymphoid Enhancer-binding Factor 1 ELISA Kit measures Lymphoid Enhancer-Binding Factor 1 in Mouse samples. The plate has been pre-coated with Mouse LEF1 antibody. LEF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LEF1 Antibody is added and binds to LEF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LEF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse LEF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2223Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
9.74 ng/L
Detection Range
20-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Transcription factor that binds DNA in a sequence-specific manner (By similarity). Participates in the Wnt signaling pathway (PubMed: 11445543). Activates transcription of target genes in the presence of CTNNB1 and EP300 (PubMed: 12446687). PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (PubMed: 11731474). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1 (By similarity). Regulates T-cell receptor alpha enhancer function (By similarity). Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (PubMed: 23562159). May play a role in hair cell differentiation and follicle morphogenesis (PubMed: 11445543). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Lymphoid Enhancer-Binding Factor 1
Regulatory Status
For Research Use Only
