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Mouse LR (Leptin Receptor) ELISA Kit
Mouse LR (Leptin Receptor) ELISA Kit
The Mouse (LR) Leptin Receptor ELISA Kit measures Leptin Receptor in Mouse samples. The plate has been pre-coated with Mouse LEPR antibody. LEPR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LEPR Antibody is added and binds to LEPR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LEPR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse LEPR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0650Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.022 ng/mL
Detection Range
0.05-30 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Metabolism, Hormone Metabolism
Background
Receptor for hormone LEP/leptin (Probable) (PubMed: 11861497). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS (PubMed: 10799542, PubMed: 25383904, PubMed: 11923481, PubMed: 11861497). In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones (PubMed: 10660043, PubMed: 12594516). In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity (PubMed: 25383904, PubMed: 11923481). Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis (PubMed: 12594516). Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus (PubMed: 25383904). Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T-cells. Leptin increases Th1 and suppresses Th2 cytokine production (PubMed: 9732873). [Isoform A]: May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis (PubMed: 17620316, PubMed: 20223942). Does not induce phosphorylation of and activate STAT3 (PubMed: 11923481, PubMed: 20223942). [Isoform E]: Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Leptin Receptor
Regulatory Status
For Research Use Only
