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Mouse LTA4H (Leukotriene A4 Hydrolase) ELISA Kit
Mouse LTA4H (Leukotriene A4 Hydrolase) ELISA Kit
The Mouse (LTA4H) Leukotriene A4 Hydrolase ELISA Kit measures Leukotriene A4 Hydrolase in Mouse samples. The plate has been pre-coated with Mouse LTA4H antibody. LTA4H present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LTA4H Antibody is added and binds to LTA4H in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LTA4H antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse LTA4H. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1583Mo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Mouse
Sensitivity
0.027 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities (By similarity). Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4) (PubMed: 1881903, PubMed: 9287304). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides (By similarity). In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of Matrix Metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL) (PubMed: 20813919). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Leukotriene A4 Hydrolase
Regulatory Status
For Research Use Only
