The Mouse (MARC1) Mitochondrial Amidoxime-Reducing Component 1 ELISA Kit measures Mitochondrial Amidoxime-Reducing Component 1 in Mouse samples. The plate has been pre-coated with Mouse MTARC1 antibody. MTARC1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MTARC1 Antibody is added and binds to MTARC1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MTARC1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MTARC1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundCatalyzes the reduction of N-oxygenated molecules, acting as a counterpart of cytochrome P450 and flavin-containing monooxygenases in metabolic cycles. As a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability. May be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis. Postulated to cleave the N-OH bond of N-hydroxylated substrates in concert with electron transfer from NADH to cytochrome b5 reductase then to cytochrome b5, the ultimate electron donor that primes the active site for substrate reduction. Source: UniProt Consortium (2025)
