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Mouse MYLK (Myosin Light Chain Kinase, Smooth Muscle) ELISA Kit

Mouse MYLK (Myosin Light Chain Kinase, Smooth Muscle) ELISA Kit

The Mouse (MYLK) Myosin Light Chain Kinase, Smooth Muscle ELISA Kit measures Myosin light chain kinase, smooth muscle in Mouse samples. The plate has been pre-coated with Mouse MYLK antibody. MYLK present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse MYLK Antibody is added and binds to MYLK in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MYLK antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse MYLK. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2281Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 5.41 ng/L
Detection Range 10-2000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Enzyme & Kinase
Background Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Myosin light chain kinase, smooth muscle
Regulatory Status For Research Use Only
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