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Mouse NE (Neutrophil Elastase) ELISA Kit

Mouse NE (Neutrophil Elastase) ELISA Kit

The Mouse (NE) Neutrophil Elastase ELISA Kit measures Neutrophil Elastase in Mouse samples. The plate has been pre-coated with Mouse ELANE antibody. ELANE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse ELANE Antibody is added and binds to ELANE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ELANE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse ELANE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0025Mo
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Mouse
Sensitivity 0.052 ng/mL
Detection Range 0.1-40 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity). Capable of killing E.coli; probably digests outer membrane protein A (ompA) in E.coli (PubMed: 10947984). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Neutrophil Elastase
Regulatory Status For Research Use Only
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