The Mouse (NLRP3) Nacht, LRR and Pyd Domains-containing Protein 3 ELISA Kit measures Nacht, Lrr and Pyd Domains-Containing Protein 3 in Mouse samples. The plate has been pre-coated with Mouse NLRP3 antibody. NLRP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NLRP3 Antibody is added and binds to NLRP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NLRP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NLRP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Product Details
Species Reactivity
Mouse
Sensitivity
1.66 ng/L
Detection Range
3-900 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology
Background
As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (or possibly CASP4/CASP11). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu (PubMed: 28847925, PubMed: 27374331). Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed: 22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K(+) efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (By similarity). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (PubMed: 26098997). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Nacht, Lrr and Pyd Domains-Containing Protein 3
Regulatory Status
For Research Use Only
