The Mouse (NOD2) Nucleotide-binding Oligomerization Domain-containing Protein 2 ELISA Kit measures Nucleotide-binding oligomerization domain-containing protein 2 in Mouse samples. The plate has been pre-coated with Mouse NOD2 antibody. NOD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NOD2 Antibody is added and binds to NOD2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOD2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NOD2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundInvolved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response (PubMed:22607974).
Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages (PubMed:18511561).
Component of an autophagy-mediated antibacterial pathway together with ATG16L1. Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteNucleotide-binding oligomerization domain-containing protein 2
