The Mouse (NOTCH1) Neurogenic Locus Notch Homolog Protein 1 ELISA Kit measures Neurogenic Locus Notch Homolog Protein 1 in Mouse samples. The plate has been pre-coated with Mouse NOTCH1 antibody. NOTCH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NOTCH1 Antibody is added and binds to NOTCH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOTCH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NOTCH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasNeuroscience, Epigenetics and Nuclear Signaling, Stem Cells
BackgroundFunctions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Source: UniProt Consortium (2025)
