The Mouse (NRF2) Nuclear Factor Erythroid 2-related Factor 2 ELISA Kit measures Nuclear Factor Erythroid 2-Related Factor 2 in Mouse samples. The plate has been pre-coated with Mouse NFE2L2 antibody. NFE2L2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse NFE2L2 Antibody is added and binds to NFE2L2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NFE2L2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Mouse NFE2L2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundTranscription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles (PubMed: 9240432, PubMed: 9887101, PubMed: 12032331, PubMed: 14517554, PubMed: 31398338). In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex (PubMed: 15282312, PubMed: 15367669, PubMed: 15581590). In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes (PubMed: 12032331). The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (PubMed: 20421418, PubMed: 20173742). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity). Source: UniProt Consortium (2025)
